On-going water sampling has been undertaken by SARDI from algal bloom sites across South Australia in 2025-26. DNA metabarcoding libraries of environmental samples have been prepared targeting the 28S rRNA gene, and next-generation sequencing used to identify species and evaluate changes in algal community dynamics. Initial samples were collected in August 2025, with repeated sampling of >10 sites from September 2025 to January 2026 used to assess seasonal succession patterns from spring to early summer. The partial length 28S rRNA gene has also been amplified and sequenced for pure culture isolates, providing a short-read reference DNA catalogue of isolates cultured from the bloom. The metabarcoding approach provides a powerful tool in tracking algal bloom dynamics, with the ability to detect species that may be overlooked or destroyed during sample preparation for microscopy, along with species that are morphologically indistinct. This method has also uncovered other species interactions, including parasitism (e.g. Amoebophyra), and has aided understanding of the role of beneficial/competing microorganisms in the bloom dynamics. Coupling DNA metabarcoding with environmental data will further assist in creating more accurate models to predict when conditions are favourable for a bloom to occur.