In mid-2025, in partnership with Bioplatforms Australia and the Australian Microbiome Initiative, IMOS launched a new environmental DNA (eDNA) sub-facility within the Biomolecular Observing Facility, opening a powerful new way to observe Australia’s marine biodiversity. For more than a decade, the IMOS Marine Microbiome Initiative has collected water samples from National Reference Stations around Australia to study microbial and planktonic communities. Building on this foundation and years of collaboration and planning, the eDNA Sub-Facility expands these observations to recover detailed data from larger marine organisms such as fish and invertebrates, using both archived and newly collected samples. This approach offers a unique opportunity to reconstruct past and present biodiversity across the tree of life, developing long-term, integrated biodiversity time series. Guided by FAIR data principles, all eDNA data will be standardised, openly accessible, and reusable. By combining eDNA with IMOS’s extensive physical and biological observations, this initiative provides a powerful new layer for detecting ecosystem change, informing marine management, and supporting conservation and sustainable ocean use. In this presentation, I will highlight progress from the first year of the sub-facility and outline how integrating archived and future samples enables scalable, long-term biodiversity monitoring across Australia’s marine environments.