Seagrass restoration for many species including Zostera muelleri rely on seed based restoration, involving the harvest of spathes and the natural release of seeds into capture nets. The age and origin of spathes can have significant impacts on collection success and the donor meadow. Therefore, we tested the effects of Z. muelleri spathe location and spathe maturity on seed production in Central Queensland, Australia. Spathes were split by location (Pelican Banks, Lilies Beach, Rods Bay, and mixed) and age (early, middle, and late stage), placed within full strength saltwater, and observed bimonthly for six months. 183 seeds were harvested in total, with 90% of the seeds collected in the first month of monitoring. Early spathes produced zero seeds, middle stage spathes produced only 16 seeds, compared to late stage spathes, which produced 167 seeds. Preliminary statistical analyses found that seed presence is determined by spathe stage and by date, with late stage spathes having significantly higher seed output versus medium stage spathes (p = 0.0155). The results of this experiment may contribute to the improved selection of spathes, limiting impacts to healthy meadows and increasing the efficiency of seed collection efforts.