Gamay (Botany Bay) is Sea Country for the La Perouse Aboriginal Community, with deep cultural, spiritual and ecological significance. For millennia, Saltwater People have observed, used and cared for this estuary, building detailed knowledge of habitats, species, seasonal cycles and ecological connectivity. Since colonisation, Gamay has been profoundly transformed by ports, airport runways, dredging and shoreline armouring – altering shorelines, water movement, habitats and access, and changing how Country functions and how people can interact with it. Gamay Seascapes ARC Linkage Project is a collaboration with the Gamay Rangers, Fisheries NSW, the University of Sydney and the University of New South Wales that aims to work collaboratively and combine Traditional Owner observations with quantitative seascape ecology to deepen understanding of this highly valued waterway. Baited remote underwater video (BRUV) is a core method used to assess fish assemblages across the diverse array of habitats in Gamay – with Fisheries NSW and Gamay Rangers having completed more than 400 BRUV drops over the past two years. Here, we use this BRUV imagery to discuss Sea Country knowledge, community stories and scientific monitoring, and discuss the ecological changes that have occurred in one of Australia’s most heavily modified estuaries.